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For the chemical method, there are many different kits used for extraction, and selecting the correct one will save time on kit optimization and extraction procedures. PCR sensitivity detection is considered to show the variation between the commercial kits. Cellular and histone proteins bound to the DNA can be removed either by adding a protease or by having precipitated the proteins with sodium or ammonium acetate , or extracted them with a phenol-chloroform mixture prior to the DNA-precipitation. After isolation, the DNA is dissolved in a slightly alkaline buffer, usually in a TE buffer , or in ultra-pure water. Some of the most common DNA extraction methods include organic extraction , Chelex extraction , and solid phase extraction. When selecting a DNA extraction method, there are multiple factors to consider, including cost, time, safety, and risk of contamination. Organic extraction involves the addition of and incubation in multiple different chemical solutions; [3] including a lysis step, a phenol chloroform extraction, an ethanol precipitation , and washing steps. Organic extraction is often used in laboratories because it is cheap, and it yields large quantities of pure DNA. Though it is easy, there are many steps involved, and it takes longer than other methods. It also involves the unfavorable use of the toxic chemicals phenol and chloroform , and there is an increased risk of contamination due to transferring the DNA between multiple tubes.

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This Density and specific gravity lab report - We will talk about this is available. Geotechnical engineering, also known as geotechnics, is the branch of civil engineering concerned with the engineering behavior of earth materials. It uses the principles and methods of soil mechanics and rock mechanics for the solution of engineering problems and the design of engineering works. Specific Gravity is the ratio of the density of the substance to the density of water. The rest of the compacted soil was broken to U. We offer you a free plagiarism report with every project delivered to you thus ensuring the writing is authentic and original. Determination of Redusing Sugar Using Dns Method

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