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Biochemical Tests On The Gram Negative And - for

For the identification of bacteria, certain general characteristics are of primary importance for determining the major group to which the new isolate is most likely to belong. Know about the nature of organism: whether it is fastidious, non-fastidious, phototropic, heterotrophic, aerobic, anaerobic, microaerophilic or facultative, its morphological features like gram reaction, endospores, capsule, rod, cocci, curved, spiral, acid fastness, motility, arrangements of cell occurring pairs, chains, groups or in packets. Score the isolate for physiological traits like catalase, oxidase, glucose oxidation-fermentation reaction, and nitrate reduction. After preliminary analysis, fit the organism in a particular group and then apply group-specific tests to identify the genus and species. There are two reasons to study the biochemical characteristics of organisms:. This test determines the ability of microorganisms to produce catalase that degrades hydrogen peroxide. During respiration, many microorganisms produce hydrogen peroxide and other reactive oxygen intermediates like superoxide and halite ions. Accumulation of these is toxic unless inactivated enzymatically. The enzyme catalase inactivates highly toxic H 2 O 2 to water and oxygen. Lack of catalase is a diagnostic characteristic of Streptococci and anaerobes. Biochemical Tests On The Gram Negative And

Biochemical Tests On The Gram Negative And Video

Identification of bacteria using biochemical tests (1 of 2)

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