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Introduction Bacteria are microscopic unicellular prokaryotic organisms characterized by the lack of a membrane-bound nucleus and membrane-bound organelles. They are remarkably adaptable to diverse environmental conditions and are found in bodies of all living organisms and on all parts of the earth.

The purpose of microbial biochemical tests is to identify the unique traits it yields and with that knowledge we can then categorize them in groups and specify them by scientific name. In order for these tests to produce reliable and credible results, the bacterium organism must be grown using strict and meticulous procedure to produce viable colonies of pure culture.

Having pure culture is significant to ensure that a single type of bacteria is used for identification without contamination so tests can be run without complications or confusion.

Once all these tests Identification Of An Unknown Bacterium performed, the unknown bacteria in this lab will be one of the following: Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumoniae, or Salmonella typhimurium. This report included the results and details to these experiments which are discussed further on. Abstract Gram negative bacteria Unknown 12 was run through an array of tests which produced positive and negative results. The results obtained from the Microbiology is requires not only an academic understanding of the microscopic world but read more a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The proper identification of a microorganism is not only important in a microbiology lab but also in the medical, industrial, and pharmaceutical fields.

Biotechnologists can also exploit the activities of microbes to benefit humans, such as in the production of medicines, enzymes and food. The goal of this lab report is 1 to demonstrate comprehension of the methods and lab techniques learned during the semester 2 to explain the tests performed on each Identiification unknown that led to the identification of each unknown 3 and to give a background on the characteristics, pathogenicity and some uses of one of the identified unknowns.

Introduction In this lab report I will discuss how I came to find my two unknown bacteria.

Each bacteria have undergo many different test to eventually Abstract In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified some unidentified bacteria based on their physical structure, colony Identification Of An Unknown Bacterium, optimal conditions and metabolic properties. The stains revealed that the bacteria were both Gram-negative and Gram-positive bacilli. Introduction A series of tests was completed in order to identify an unknown microorganism. A gram stain reaction test was done. A gram stain reaction test is used to differentiate between two bacterial species. The two species; gram positive and gram negative bacteria have varying properties of their cell wall structural composition.

The gram positive bacteria contain a thick peptidoglycan layer in their cell wall which retains the primary crystal violet stain. The crystal violet is washed from the fixed stain and gram-negative bacteria appear red after the decolorizer washes the primary stain due to their more porous higher lipid content walls and the safranin counter stain adheres to their thinner cell wall. The microscopic examination of the bacteria after staining allows for the morphology of the organism to be determined because as the cell is killed during Identification Of An Unknown Bacterium staining process it retains its rigid structure allowing for morphology determination.

A fermentation test was done. Three different carbohydrates are used to determine whether the organism can ferment a sugar as well as if a gas is produced during Bacterlum. A phenol red broth is used which retains a red color at a pH of 7.

When an acid is Identification Of An Unknown Bacterium during fermentation, the pH of the broth will lower and the broth will turn yellow. The sugars Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing.]