Human Papillomaviruses Hpvs Video
Natural History of HPV InfectionHuman Papillomaviruses Hpvs - opinion
Cervical cancer is the fourth-leading cause of cancer deaths among women worldwide and most cases occur in developing countries. Detection of high-risk HR HPV, the etiologic agent of cervical cancer, is a primary screening method for cervical cancer. However, the current gold standard for HPV detection, real-time PCR, is expensive, time-consuming, and instrumentation-intensive. A rapid, low-cost HPV detection method is needed for cervical cancer screening in low-resource settings. We previously developed a digital loop-mediated isothermal amplification dLAMP assay for rapid, quantitative detection of nucleic acids without the need for thermocycling. This assay employs a microfluidic self-digitization chip to automatically digitize a sample into an array of nanoliter wells in a simple assay format. Here we evaluate the dLAMP assay and self-digitization chip for detection of the commonly tested 14 high-risk HPVs in clinical samples.
Human Papillomaviruses Hpvs.
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Clicking on the donut icon will load a page at altmetric. Find more information on the Altmetric Attention Score and how the score is calculated. Cervical cancer Papillomavirusee the fourth-leading cause of cancer deaths among women worldwide and most cases occur in developing countries. Detection of high-risk HR HPV, the etiologic agent of cervical cancer, is a Human Papillomaviruses Hpvs screening Human Papillomaviruses Hpvs for cervical cancer.
However, the current gold standard for HPV detection, real-time PCR, is expensive, time-consuming, and instrumentation-intensive. A rapid, low-cost HPV detection method is needed for cervical cancer screening in low-resource settings. We previously developed a digital loop-mediated isothermal amplification dLAMP assay for rapid, quantitative detection of nucleic acids without the need for thermocycling.
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This assay employs a microfluidic self-digitization chip to automatically digitize a sample into an array of nanoliter wells in a simple assay format. Here we evaluate the dLAMP assay and self-digitization chip for detection of the commonly tested 14 high-risk HPVs in clinical samples.
The dLAMP platform provided reliable genotyping and quantitative detection of the 14 high-risk HPVs with high sensitivity, demonstrating its potential for simple, rapid, and low-cost diagnosis of HPV infection. The Cervical swab samples. Optimization of dLAMP digital efficiency. The American Chemical Society holds a copyright ownership interest in any copyrightable Supporting Information.
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