Lab Report Bacillus Subtilus Using A Polymerase Video
Microbiology YouTube Project: amazonia.fiocruz.bris Lab Report Bacillus Subtilus Using A PolymeraseBasic Info
The secretory production of bacterial AFs has not been attempted previously. In this study, we designed a unique induction system for the production of a recombinant AF in Bacillus subtilis in On Anita Desai to exploit its enzymic degradation of wheat bran. We found that non-starch phytochemicals were more efficient than d-xylose when inducing the expression of T7 RNA polymerase and driving the transcription of AF by the T7 promoter. The host cell, B. When cultured in a flask with gentle shaking, and with d-xylose as an inducer, the recombinant strain successfully expressed arbf, a family 51 GH 51 glycoside hydrolase from Bacillus licheniformis, and secreted However, the protein was devoid of a secretary signal peptide.
When cultures were supplemented with wheat bran, the maximal yield of the secreted AF reached The results provide a foundation for the high level production of heterologous proteins using wheat bran as the inducer in B.]
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