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You will learn about modern analytical techniques and limitations as applied in clinical laboratories. Topics include the basic theory and applications of analytical techniques that are commonly used in clinical laboratories. Pak-Cheung Chan pc. LMPH: Basic principles of analytical clinical biochemistry. Course description You will learn about modern analytical techniques and limitations as applied in clinical laboratories. Course coordinator Dr. Timings and location This course is offered every year in the Fall session. Wednesdays, a. Basic Principles Of A Chemistry Lab

Basic Principles Of A Chemistry Lab Video

Le Chatelier's Principle of Chemical Equilibrium - Basic Introduction

Chromatography is yet another technique for the analysis and separation of chemical mixtures. The technique is based on a polarity interplay between the sample and two other substances called the solid or stationary phaseand the mobile phase, which can be a liquid or a gas. As their names indicate, the stationary phase does not move, whereas the mobile phase flows across, or through the solid phase.

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More polar samples adhere or bind more strongly than less polar ones. The mobile phase then comes in and sweeps across the stationary phase, competing for the sample. If the mobile phase is a solvent, it is called the eluting solvent.

Different components with different polarities will travel at different rates as the solvent moves, causing their separation. Some of the most common solid adsorbents used as stationary phases in chromatography are listed in Basiv In the organic chemistry lab the most commonly used are silica gel and alumina due to their versatile polarity range. Likewise, some common eluting solvents are listed in table Methylene chloride, ethyl acetate, and ethanol are among the most commonly used.

Table Again, the more polar ones alcohols, amines, and acids move slower, whereas the less polar ones hydrocarbons and ethers move faster.]