Restriction Digest Involves The Uses Of Restriction - amazonia.fiocruz.br

Restriction Digest Involves The Uses Of Restriction - think

Methylation can change the activity of a DNA segment without changing the sequence. When located in a gene promoter , DNA methylation typically acts to repress gene transcription. In mammals, DNA methylation is essential for normal development and is associated with a number of key processes including genomic imprinting , X-chromosome inactivation , repression of transposable elements , aging , and carcinogenesis. Two of DNA's four bases, cytosine and adenine , can be methylated. Methylation of cytosine to form 5-methylcytosine occurs at the same 5 position on the pyrimidine ring where the DNA base thymine 's methyl group is located; the same position distinguishes thymine from the analogous RNA base uracil , which has no methyl group. Spontaneous deamination of 5-methylcytosine converts it to thymine. This results in a T:G mismatch. Repair mechanisms then correct it back to the original C:G pair; alternatively, they may substitute A for G, turning the original C:G pair into a T:A pair, effectively changing a base and introducing a mutation.

Restriction Digest Involves The Uses Of Restriction Video

Restriction Map and Gel Electrophoresis Restriction Digest Involves The Uses Of Restriction

More Information:

Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Boyer and G. Yannic and D. Rioux and A. Next-generation sequencing technologies have opened a new era of Ibvolves in population genetics. Following these new sequencing opportunities, the use of restriction enzyme-based genotyping techniques, such as restriction site-associated DNA sequencing RAD-seq or double-digest RAD-sequencing ddRAD-seqhas dramatically increased in the last decade.

Our Related Sites

View on Springer. Save to Library. Create Alert. Launch Research Feed.

Introduction

Share This Paper. Related Papers. Abstract Related Papers. By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License.]