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Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition.

Research is underway to develop various sources for stem cells as well as to apply stem-cell treatments for neurodegenerative diseases [3] and conditions such as diabetes and heart disease. Stem-cell therapy has become controversial Crjtique developments such as the ability of scientists to isolate and culture embryonic stem cellsto create stem cells using somatic cell nuclear transfer and their use of techniques to create induced pluripotent stem cells.

This controversy is often related to abortion politics and to human cloning. Additionally, efforts to market treatments based on transplant of stored umbilical cord blood have been controversial. For over 30 years, hematopoietic stem cell transplantation HSCT has been used to treat people with conditions such as leukaemia and lymphoma ; this is the only widely practiced form of stem-cell therapy. These agents, however, cannot discriminate between the leukaemia or neoplastic cells, and the hematopoietic stem cells within read article bone marrow. This is the side effect of conventional chemotherapy strategies tor the stem-cell transplant attempts to reverse; a donor's healthy bone marrow reintroduces functional stem cells to replace the cells lost in the host's body during treatment.

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The transplanted cells also generate an immune response that helps to kill off the cancer cells; this process can go too far, however, leading to graft vs host diseasethe most serious side effect of this treatment. Another stem-cell therapy, called Prochymalwas conditionally approved in Canada in for the management of acute graft-vs-host disease in children who are unresponsive to steroids. MSCs are purified from the marrow, cultured and packaged, with up to 10, doses derived from a single donor. The doses are stored frozen until needed. The FDA has approved five hematopoietic stem-cell products derived from umbilical-cord blood, for the treatment of blood and immunological diseases. Inthe European Medicines Agency recommended approval of limbal stem cells for people with severe limbal stem cell deficiency due to burns in the eye. Stem cells are being studied for Critique on Damages as a Remedy for number of reasons.

The molecules and exosomes released from stem cells are also being studied in an effort to make medications. To be used for research or treatment applications, large numbers of high-quality stem cells are needed.

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It is a necessary requirement to develop a culture system to produce pure populations of tissue-specific stem-cells in vitro without the loss of stem-cell potential. Two main approaches are taken for this purpose - two-dimensional and three-dimensional cell culture. Cell culture in two dimensions has been routinely performed in thousands of laboratories worldwide for the past four decades. In two-dimensional platforms, cells are typically exposed to a solid, rigid flat surface on the basal side and to liquid at the apical surface.

Inhabiting such a two-dimensional rigid substrate requires a dramatic adaption for the surviving cells because they lack the extracellular matrix that is unique to each cell type, and which may alter cell metabolism and reduce its functionality.

Background

Three-dimensional cell culture systems may create a biomimicking microenvironment for stem cells, resembling their native three-dimensional extracellular matrix ECM. Advanced biomaterials have significantly contributed to three-dimensional cell culture systems in recent decades, and more unique and complex biomaterials have been proposed for improving stem-cell proliferation and controlled differentiation. Among them, nanostructured biomaterials are of particular interest because they have the advantage of a high surface-to-volume ratio, and they mimic the physical and biological features of natural ECM at the nanoscale. Research has been conducted on the effects of stem cells on animal models of brain degenerationsuch as in Parkinson's diseaseAmyotrophic lateral sclerosisand Alzheimer's disease.

Healthy adult brains learn more here neural stem cellsCritique on Damages as a Remedy for divide to maintain general stem-cell numbers, or become progenitor cells. Cfitique healthy adult laboratory animals, progenitor cells migrate within the brain and function primarily to maintain neuron populations for olfaction the sense of smell. Pharmacological fof of endogenous neural stem cells has been reported to induce neuroprotection and behavioral recovery in adult rat models of neurological disorder. Stroke and traumatic brain injury lead to cell deathcharacterized by a loss of neurons and oligodendrocytes within the brain.]