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Dynamic Biological Processes Influence Pop Density Dispersion. Dynamic Biological Processes Influence Pop Density Dispersion

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In MRI and NMR spectroscopyan observable nuclear spin polarization magnetization is created by an RF pulse or a train of pulses applied to a sample in a homogeneous Diepersion field at the resonance Larmor frequency of the nuclei. At thermal equilibrium, nuclear spins precess randomly about the direction of the applied field but become abruptly phase coherent when any of the resultant polarization is created orthogonal to the field.

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This transverse magnetization can induce a signal in an RF coil that can be detected and amplified by an RF receiver. The RF pulses cause the population of spin-states to be perturbed from their thermal equilibrium value. The return of the longitudinal component of the magnetization to its equilibrium value is termed spin-lattice relaxation while the loss of phase-coherence of the spins is termed spin-spin relaxation, which is manifest as an observed free induction decay FID.

The energy gap between the spin-up and spin-down states in NMR is minute by atomic emission standards at magnetic fields conventionally used in MRI and NMR spectroscopy. Energy emission in NMR must be induced through a direct interaction of a nucleus with its external environment rather than by spontaneous emission. This interaction may be through the electrical or magnetic fields generated by other nuclei, electrons, or molecules.

Spontaneous emission of energy is a radiative process involving the release of a photon and typified by phenomena Dynamic Biological Processes Influence Pop Density Dispersion as fluorescence and phosphorescence. The decay of RF-induced NMR spin polarization is characterized in terms of two separate processes, each with their own time constants.

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One process, called T 1is responsible Dispesrion the loss of resonance intensity following pulse excitation. The other process, called T 2characterizes the width or broadness of resonances. Stated more formally, T 1 is the time constant for the physical processes responsible for the relaxation of the components of the nuclear spin magnetization vector M parallel to the external magnetic field, B 0 which is conventionally designated as the z -axis.

T 2 relaxation affects the coherent components of M perpendicular to B 0.

Dynamic Biological Processes Influence Pop Density Dispersion

Values of T 1 range from milliseconds to several seconds, depending on the size of the molecule, the viscosity of the solution, the temperature of the sample, and the possible presence of paramagnetic species e. In general. T 1 relaxation involves redistributing the populations of the nuclear spin states in order to reach the thermal equilibrium distribution. By definition, this is not energy conserving. Moreover, spontaneous emission is negligibly slow at NMR frequencies. Hence truly isolated nuclear spins would show negligible rates of T 1 relaxation. However, a variety of relaxation mechanisms allow nuclear spins to exchange energy with their surroundings, Dynamic Biological Processes Influence Pop Density Dispersion latticeallowing the spin populations to equilibrate.

The fact that T 1 relaxation involves an interaction with the surroundings is the origin of the alternative description, spin-lattice relaxation. Note that the rates of T 1 relaxation i. Small amounts of paramagnetic substances in a sample speed up relaxation very much. Especially Dispersiom molecules exhibiting slowly relaxing T 1 signals, the technique spin saturation transfer SST provides information on Procfsses exchange reactions.

The method is widely applicable to fluxional molecules.

For instance, initial xy magnetization at time zero will decay to zero i. T 2 relaxation is a complex phenomenon, but at its most fundamental level, it corresponds to a decoherence of the transverse nuclear spin magnetization. Random fluctuations of the local magnetic field lead to random variations in the instantaneous NMR Biologicwl frequency of different spins.

Dynamic Biological Processes Influence Pop Density Dispersion

As a result, the initial phase coherence of the nuclear spins is lost, until eventually the phases are disordered and there is no net xy magnetization. Because T 2 relaxation involves only the phases of other nuclear spins it is often called "spin-spin" relaxation. T 2 values are generally much less dependent on field strength, B, than T 1 values.

Hahn echo decay experiment can be Dsipersion to measure the T 2 time, as shown in the animation below. The size of the echo is recorded for different spacings of the two applied pulses. In an idealized system, all nuclei in a given chemical environment, in a magnetic field, precess with the same frequency. However, in real systems, there are minor differences in chemical environment which can lead to a distribution of resonance frequencies around the ideal.]

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