Elucidation Of The Working Concentration Of Kanamycin - amazonia.fiocruz.br

Elucidation Of The Working Concentration Of Kanamycin Video

Metrics details. Diabetes is a complex metabolic disease characterized by hyperglycemia, plaguing the whole world. However, the action mode of multi-component and multi-target for traditional Chinese medicine TCM could be a promising treatment of diabetes mellitus.

Top Life Science Categories

Up to now, its hypoglycemic active ingredients and mechanism of action are not yet clear. In this study, network pharmacology was employed to elucidate the potential bioactive compounds and hypoglycemic Kanamyin of PM. First, the compounds see more good pharmacokinetic properties were screened from the self-established library of PM, and the targets of these compounds were predicted and collected through database. Relevant targets of diabetes were summarized by searching database.

The intersection targets of compound-targets and disease-targets were obtained soon. Secondly, the interaction net between the compounds and the filtered targets was established. These key targets were enriched and analyzed by protein—protein interactions PPI analysis, molecular docking verification.

Thirdly, the key genes were used to find the biologic pathway and explain the therapeutic mechanism by genome ontology GO and kyoto encyclopedia of genes and genomes KEGG analysis. Lastly, the part of potential bioactive compounds were under enzyme activity inhibition tests.

Suggested Reading

In this study, 29 hypoglycemic components and 63 hypoglycemic targets of PM were filtrated based on online network database. Then the component-target interaction network was constructed and five key components resveratrol, apigenin, kaempferol, quercetin and luteolin were further obtained. Docking studies indicated that the bioactive compounds could stably bind the pockets of target proteins. There were 38 metabolic pathways, including regulation https://amazonia.fiocruz.br/scdp/blog/woman-in-black-character-quotes/african-american-cultural-values-and-norms.php lipolysis in adipocytes, prolactin signaling pathway, TNF signaling pathway, VEGF signaling pathway, FoxO signaling pathway, estrogen signaling pathway, linoleic acid metabolism, Rap1 signaling pathway, arachidonic acid metabolism, and osteoclast differentiation closely connected with the hypoglycemic mechanism of PM.

In summary, the study used systems pharmacology to elucidate the main hypoglycemic components and mechanism of PM. The work provided a scientific basis for the further hypoglycemic effect research of PM and its monomer components, but also provided a reference for the secondary development of PM.

1. Introduction

It is generally believed that diabetes is caused by a lack of insulin secretion activity or insulin resistance. Usually diabetes can be simply divided into type 1 diabetes mellitus T1DM and type 2 diabetes mellitus T2DM due to different pathogenesis. As a result of the long-term existence of hyperglycemia, it often accompanied by a variety of diabetic complications [ 1 ], such as diabetes cardiomyopathy [ 2 ], diabetic ketoacidosis [ 3 ], diabetic gastroparesis [ 4 ], retinopathy [ 5 ], diabetic nephropathies [ 6 ]. These diabetes-related diseases not only have a huge impact on the body and spirit of the patients, but also bring great pressure to society.

Up to now, a great deal of effort and much money have been devoted to developing drugs to treat diabetes, and many drugs have been successfully developed for different targets. Some drugs targeting glucosidase [ 8 ], glucagon-like peptide-1 glp-1 receptor [ 9 ], dipeptidase IV dpp-4 [ 10 ], and sodium glucose co-transporter 2 SGLT2 [ 11 ] and the like have been already available on market [ 12 ]. Traditional Chinese medicine TCM is considered as a complex system due to its intricate components, which correspond to complex organic targets. The system network based on the interaction between multiple components and multiple targets will help to illuminate the complicated mechanism of TCM in the treatment of diseases.]